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Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles

Mitiku Diriba Jobir, Abera Belay Shume.

Abstract
Introduction: Propolis is one of the natural hive products and a sticky material mixed by honeybees to utilize it in protecting their hives from infection by bacteria and fungi.
Aim: The objective of this study was to investigate the wound healing, antioxidant, antibacterial, physicochemical and mineral profiles of propolis collected from three different region of Ethiopia: Asela, Sheka and Gambela.
Methods: Physicochemical properties and mineral profile determined using standard analytical methods, antibacterial examination was determined using disc diffusion method, antioxidant properties examined using spectrophotometric method, and rodent model used to investigate the wound healing property of propolis.
Results: Ethiopian propolis had physicochemical properties for moisture (3.64-6.69%), ash (2.05-3.54%), soluble substance (45.28-71.85%), insoluble substances (28.15-54.74%), saponification value (82.27-127.89), conductivity (0.13-0.24), pH (4.82-5.49), waxes (7.80-8.89%), acid value (10.07-35.14), ester value (63.84-107.03), melting point (63.03-83.95). Minerals found in propolis were Na, Ca, Mg, K, Fe, Zn, Cu and Co. The inhibition zone (mm) for the antibacterial property range from 6.55±0.20 -10.21±0.16 (water extract) and 7.09±0.06 -12.06±0.03 (ethanol extracts). The phenolic content of Asela, Sheka and Gambela propolis were 63.09±3.55, 72.26±7.65 and 82.07±3.72mgGAE/g, respectively. The flavonoid content of propolis for Asela, Sheka and Gambela were 24.42±0.53, 17.26±0.35 and 22.68±0.94mgQE/g, respectively; these help to have good antioxidant activities. Inhibition concentration (IC50) values of DPPH for Asela, Sheka, Gambela propolis and ascorbic acid were 18.13±0.002, 12.17±0.002, 22.07±0.001 and 15.20±0.002 (μgmL-1), respectively. There was a significant variation between the four treatments. Asela, Sheka and Gambela propolis wound area (mm2) at the 14th day was 0.51±0.01, 0.60±0.01, 0.49±0.01, respectively. The highest wound improvement (%) was 84.49±0.20 (Gambela propolis) followed by Asela (83.75±0.33) and Sheka propolis (80.88±0.32).
Conclusion: Propolis collected from Ethiopia had varied wound healing, antioxidant, antibacterial, physicochemical and mineral profile. These properties can make propolis a good candidate in food, pharmaceutical industries and traditional healing practices.

Key words: Propolis, Wound healing, Antibacterial, Antioxidant, Physicochemical, Minerals


 
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Pubmed Style

Jobir MD, Shume AB. Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850


Web Style

Jobir MD, Shume AB. Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. http://www.japitherapy.com/?mno=88997 [Access: June 24, 2020]. doi:10.5455/ja.20200225125850


AMA (American Medical Association) Style

Jobir MD, Shume AB. Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850



Vancouver/ICMJE Style

Jobir MD, Shume AB. Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850



Harvard Style

Jobir, M. D. & Shume, . A. B. (2020) Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850



Turabian Style

Jobir, Mitiku Diriba, and Abera Belay Shume. 2020. Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850



Chicago Style

Jobir, Mitiku Diriba, and Abera Belay Shume. "Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles." doi:10.5455/ja.20200225125850



MLA (The Modern Language Association) Style

Jobir, Mitiku Diriba, and Abera Belay Shume. "Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles." doi:10.5455/ja.20200225125850



APA (American Psychological Association) Style

Jobir, M. D. & Shume, . A. B. (2020) Comparative Study of Different Ethiopian Propolis: In Vivo Wound Healing, Antioxidant, Antibacterial, Physicochemical –Properties and Mineral Profiles. doi:10.5455/ja.20200225125850





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