Alteration of PUMA gene expression in mice by royal jelly and green tea


Wael S. Abdel-Mageed, Rasha Fathy, Mohamed Othman

Aim: The purpose of this study examine whether the polymorphism in (TNF-α) gene correlated with activate p53 upregulated modulator of apoptosis (PUMA) gene consequential exposure to pesticides and the effects of green tea and royal jelly in rats.Methods: Experimental groups were organized into 12 groups including 10 animals per each. Contain control group, pesticide group and the rest of groups were pesticide + protective. Blood samples were collected form rats before and after occupational exposure to pesticides for liver and kidney enzymes biochemical analysis, the polymorphism of the genes for TNF-α and PUMA were typed from genomic DNA and Allelic frequencies and carriage rates were compared between healthy and treated rats. The correlation between TNF-α and PUMA genotypes and the clinical characteristics of rats were also evaluated. Results: Data statistical analysis for mitochondrial enzymes in our study showed a very highly significant (P>0.001) difference in the mean value of serum enzymes isolated from treatments groups with pesticides before and after Royal jelly and Green tea treatments. The results of restriction enzymes for TNF-αshowed there are no different between negative control with and both of two protective agents in the results of restricted bands. But the results from PUMA showed a strong deferent between the treatments with royal jelly and green tea combined with pesticides. The royal jelly and green tea enhance the repair system enzymes to remedy the mutation that originated in DNA after exposure to pesticides. Conclusion: Our recent work provides direct evidence Royal jelly and Green tea could be affects on mechanism related to the repair system in cell to remedy the DNA mutation