Md. A. Rayhan, Syed A. Yousuf, Johir Rayhan, Elmabruk M. Khengari, Kashfia Nawrin, Mohammad M. Billah

Introduction:Prophetic Medicine is a rich branch of natural prophylactic medicine recommended by the Prophet Muhammad (Peace Be Upon Him) and is scientifically proved to behighly effective against almost all types of ailments. Honey and Black cumin are the most investigated ingredients of prophetic medicine for the scientific explanation of their benefits. The present study was designed to explore the neuropharmacological potential of Honey derived from Black Seed flower.Materials and Methods: For evaluation of its neuropharmacological potential, modified open field, hole cross, and elevated plus maze (OF-HC-EPM) approach was applied on female Swiss Albino mice, which was basically an integration of OF-HC-EPM experimen-tal cascade to trial with the rodents after single oral administration where parameters for sedative and anxiolytic behavior such as square crossing, hole crossing, grooming, rearing, entry, and duration in open or close arms were observed.Results: Dose-dependent (2, 4, and 6 g/kg of body weight) analysis was performed against standard Diazepam (1 mg/kg). At low doses (2 g/kg), honey possesses anxiolytic action avoiding sedation, whereas at high doses (4 and 6 g/kg), it caused mild sedative response yet developed decision-making capability and increased confidence. On the contrary, Diazepam was associated with sedative followed by anxiolytic activity.Conclusion: From the study, it was concluded that black seed honey exhibits non-sedative anxiolytic activity in dose-dependent manner in comparison to the established standard drug where sedation is often considered a limitation. Thus, as exhibited by black seed honey, it can be a good source for new drug development