E-ISSN 2474-137X
 

Original Research 


Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey

Odangowei Inetiminebi Ogidi, Patrick Adigwe, Uchechi Emmanuella Enenebaku, Mike Noah Ayebaboagha.

Abstract
Background/Aim: Honey is one of the oldest traditional medicines considered as remedy for microbial infections. It is also recognized as an efficacious tropical antimicrobial agent in the treatment of burns and wounds. The aim of the study was to determine the antimicrobial susceptibility of ethanol, methanol and acetate extracts of processed honey.
Materials and Methods: Processed honey sample was obtained from local market in Yenagoa. Test organisms were Gram-positive (Staphylococcus aureus and Bacillus cereus,) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) bacteria and fungi (Aspergillus niger, Mucor mucaralis, Tricophyton tonsurance, Microsporum ferrogenium and Aspergillus flavus). And 10 fold serial dilutions were made and plated and antimicrobial activity was evaluated using the agar well-diffusion method.
Results: The results of antibacterial activity of the extracts against tested organisms show the diameter of inhibition zone ranging from 4 to 25mm for Staphylococcus aureus, 14.5 to 22mm for Escherichia coli, 16.9 to 20mm for Salmonella typhi, 1 to 17mm for Bacillus cereus, and 12.6 to 20mm for Pseudomonas aeruginosa. While for antifungal activity show the diameter of inhibition zone ranging from 10.2 to 15mm for Aspergillus niger, Mucor mucaralis (4 to 7mm), Tricophyton tonsurance (8 to 11mm), Microsporum ferrogenium (4 to 18mm) and Aspergillus flavus (2 to 8mm).
Conclusion: From the result test bacteria were found to be more susceptible compared to fungi. The study showed that honey has antimicrobial activity against test organisms and provides alternative therapy for the inhibition of these pathogenic microorganisms.

Key words: Acetate; Ethanol;Honey;Methanol;Microbial Sensitivity Test;


 
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Pubmed Style

Ogidi OI, Adigwe P, Enenebaku UE, Ayebaboagha MN, . Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. J Apither. 2019; 6(1): 1-5. doi:10.5455/ja.20190812081333


Web Style

Ogidi OI, Adigwe P, Enenebaku UE, Ayebaboagha MN, . Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. http://www.japitherapy.com/?mno=302644402 [Access: November 15, 2019]. doi:10.5455/ja.20190812081333


AMA (American Medical Association) Style

Ogidi OI, Adigwe P, Enenebaku UE, Ayebaboagha MN, . Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. J Apither. 2019; 6(1): 1-5. doi:10.5455/ja.20190812081333



Vancouver/ICMJE Style

Ogidi OI, Adigwe P, Enenebaku UE, Ayebaboagha MN, . Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. J Apither. (2019), [cited November 15, 2019]; 6(1): 1-5. doi:10.5455/ja.20190812081333



Harvard Style

Ogidi, O. I., Adigwe, P. . ., Enenebaku, U. E., Ayebaboagha, M. N. & (2019) Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. J Apither, 6 (1), 1-5. doi:10.5455/ja.20190812081333



Turabian Style

Ogidi, Odangowei Inetiminebi, Patrick Adigwe, Uchechi Emmanuella Enenebaku, Mike Noah Ayebaboagha, and . 2019. Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. Journal of Apitherapy, 6 (1), 1-5. doi:10.5455/ja.20190812081333



Chicago Style

Ogidi, Odangowei Inetiminebi, Patrick Adigwe, Uchechi Emmanuella Enenebaku, Mike Noah Ayebaboagha, and . "Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey." Journal of Apitherapy 6 (2019), 1-5. doi:10.5455/ja.20190812081333



MLA (The Modern Language Association) Style

Ogidi, Odangowei Inetiminebi, Patrick Adigwe, Uchechi Emmanuella Enenebaku, Mike Noah Ayebaboagha, and . "Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey." Journal of Apitherapy 6.1 (2019), 1-5. Print. doi:10.5455/ja.20190812081333



APA (American Psychological Association) Style

Ogidi, O. I., Adigwe, P. . ., Enenebaku, U. E., Ayebaboagha, M. N. & (2019) Determination of Antimicrobial Susceptibility of Ethanol, Methanol and Acetate Extracts of Processed Honey. Journal of Apitherapy, 6 (1), 1-5. doi:10.5455/ja.20190812081333





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