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Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.

Mar a Bel n Ventura, Javier Leandro Barber n, Diego Karim Yamul, Patricio Jos Leaden, Pedro Adolfo Zeinsteger, Alejandro Palacios.

Abstract
Honey is a substance produced by bees and other social insects, from nectar or molasses that they gather on living plants and that transform or elaborate by evaporating water and action of enzymes, segregated by them, being stored in the alveoli or honeycomb cells. A prior investigation indicated that the total antioxidant activity of honey is primarily provided by its phenolic composition. Polyphenols mainly exert their antioxidant activity by neutralizing free radicals. The objective of this study was to investigate the antioxidant effect of honey on the peroxidation of hepatic microsomes membranes. Rat liver microsomes were incubated with different concentrations of honey (25, 50, 100, 200 mg) in an in vitro non-enzymatic ascorbic acid-Fe+2 system in order to determine the oxidative effect on membranes and to quantify peroxidation level in standardized conditions. The microsomal peroxidation was quantified in a liquid scintillation counter Packard 1900 TR by chemiluminescence in cpm (counts per minute). Microsomal membranes without honey were used as controls. Analyzing the effect of honey was observed that the total cpm/mg protein originated from light emission: chemiluminescence, was statistically lower in samples obtained from honey group than in the control group (without honey), the antioxidant effect found was dependent concentration.

Key words: Honey, Microsomes, Antioxidants, Peroxidation, Chemiluminescence


 
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How to Cite this Article
Pubmed Style

Ventura MaBn, n JLB, Yamul DK, Leaden PJ, Zeinsteger PA, Palacios A. Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. J Apither. 2020; 7(1): 10-14. doi:10.5455/ja.20190814014320


Web Style

Ventura MaBn, n JLB, Yamul DK, Leaden PJ, Zeinsteger PA, Palacios A. Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. http://www.japitherapy.com/?mno=302644425 [Access: March 17, 2020]. doi:10.5455/ja.20190814014320


AMA (American Medical Association) Style

Ventura MaBn, n JLB, Yamul DK, Leaden PJ, Zeinsteger PA, Palacios A. Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. J Apither. 2020; 7(1): 10-14. doi:10.5455/ja.20190814014320



Vancouver/ICMJE Style

Ventura MaBn, n JLB, Yamul DK, Leaden PJ, Zeinsteger PA, Palacios A. Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. J Apither. (2020), [cited March 17, 2020]; 7(1): 10-14. doi:10.5455/ja.20190814014320



Harvard Style

Ventura, M. a. B. n., n, . J. L. B., Yamul, . D. K., Leaden, . P. J., Zeinsteger, . P. A. & Palacios, . A. (2020) Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. J Apither, 7 (1), 10-14. doi:10.5455/ja.20190814014320



Turabian Style

Ventura, Mar a Bel n, Javier Leandro Barber n, Diego Karim Yamul, Patricio Jos Leaden, Pedro Adolfo Zeinsteger, and Alejandro Palacios. 2020. Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. Journal of Apitherapy, 7 (1), 10-14. doi:10.5455/ja.20190814014320



Chicago Style

Ventura, Mar a Bel n, Javier Leandro Barber n, Diego Karim Yamul, Patricio Jos Leaden, Pedro Adolfo Zeinsteger, and Alejandro Palacios. "Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.." Journal of Apitherapy 7 (2020), 10-14. doi:10.5455/ja.20190814014320



MLA (The Modern Language Association) Style

Ventura, Mar a Bel n, Javier Leandro Barber n, Diego Karim Yamul, Patricio Jos Leaden, Pedro Adolfo Zeinsteger, and Alejandro Palacios. "Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.." Journal of Apitherapy 7.1 (2020), 10-14. Print. doi:10.5455/ja.20190814014320



APA (American Psychological Association) Style

Ventura, M. a. B. n., n, . J. L. B., Yamul, . D. K., Leaden, . P. J., Zeinsteger, . P. A. & Palacios, . A. (2020) Evaluation of the antioxidant activity of honey in the peroxidation of rat liver microsomes.. Journal of Apitherapy, 7 (1), 10-14. doi:10.5455/ja.20190814014320





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